REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ENHANCED-FLUIDITY MOBILE PHASES

被引：42

作者：

CUI, Y ^{[1
]}

OLESIK, SV ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT CHEM,COLUMBUS,OH 43210

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 691卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0021-9673(94)00878-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of enhanced-fluidity liquids in reversed-phase HPLC separations is characterized. Enhanced fluidi liquids are commonly used liquids with high proportions of low viscosity fluids, such as carbon dioxide, added. When carbon dioxide is added to the methanol-water mobile phase, substantially lower plate heights and time of analysis are achieved without losing mobile phase solvent strength. The results indicate that these improvements are caused by the combination of the increased diffusivity of the enhanced-fluidity solvents and the ability of carbon dioxide to readily break hydrogen bonds in the methanol-water mixtures.

引用

页码：151 / 162

页数：12

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