COMPARISON OF ALPHA-ACETOLACTATE SYNTHASE AND ALPHA-ACETOLACTATE DECARBOXYLASE IN LACTOCOCCUS SPP AND LEUCONOSTOC SPP

被引：31

作者：

MONNET, C ^{[1
]}

PHALIP, V ^{[1
]}

SCHMITT, P ^{[1
]}

DIVIES, C ^{[1
]}

机构：

[1] UNIV BOURGOGNE,ENS,BANA,DEPT MICROBIOL BIOTECHNOL,F-21000 DIJON,FRANCE

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 03期

关键词：

D O I：

10.1007/BF00134622

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell-free extracts of Leuconostoc and Lactococcus species were tested for their alpha-acetolactate synthase and alpha-acetolactate decarboxylase activities. In Leuconostoc mesenteroides subsp. cremoris, Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc lactis, the Km of alpha-acetolactate synthase for pyruvate was close to 10 mM whereas it was 30 mM in Lactococcus lactis subsp. lactis biovar. diacetylactis. The Km of alpha-acetolactate decarboxylase for alpha-acetolactic acid was very low (0.3 mM) in Leuconostoc species in comparison to Lactococcus lactis subsp. lactis biovar. diacetylactis (60 mM). In the latter bacterium, alpha-acetolactate decarboxylase showed a sigmoidal dependance upon alpha-acetolactic acid and was activated by the three branched-chain amino acids: leucine, isoleucine and valine.

引用

页码：257 / 262

页数：6

共 17 条

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