DIRECT AND COMPETITIVE KINETIC-ANALYSIS OF THE INTERACTION BETWEEN HUMAN IGG1 AND A ONE DOMAIN ANALOG OF PROTEIN-A

被引：23

作者：

KARLSSON, R

JENDEBERG, L

NILSSON, B

NILSSON, J

NYGREN, PA

机构：

[1] PHARMACIA BIOSCI CTR,S-11287 STOCKHOLM,SWEDEN

[2] ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM,SWEDEN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 183卷 / 01期

关键词：

KINETIC ANALYSIS; LOW MOLECULAR WEIGHT ANALYTE; BIOSENSOR;

D O I：

10.1016/0022-1759(95)00030-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct and competitive kinetic analysis of the binding between a one domain analogue of protein A, and mutants thereof, to immobilised hIgG1 was compared using a biosensor system based on surface plasmon resonance detection. Rate constants determined from both assays were almost identical. The experiments demonstrate that competitive kinetic analysis can be used in combination with biosensor technology, and indicate that competitive kinetic analysis may extend the use of the technology to include low molecular weight analytes.

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页码：43 / 49

页数：7

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