ANTIGENIC ANALYSIS OF NEMATODE-TRANSMISSIBLE AND NON-TRANSMISSIBLE ISOLATES OF TOBACCO RATTLE TOBRAVIRUS USING MONOCLONAL-ANTIBODIES

被引：3

作者：

LEGORBURU, FJ ^{[1
]}

ROBINSON, DJ ^{[1
]}

TORRANCE, L ^{[1
]}

DUNCAN, GH ^{[1
]}

机构：

[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-6-1497

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Murine monoclonal antibodies (MAbs) were produced against a nematode non-transmissible isolate of tobacco rattle tobravirus (TRV-PLB). Two of the MAbs (SCR 80 and 81) did not react with the serologically closely related isolate TRV-PPK20 (both isolates belong to the PRN serotype), which is readily transmissible by When further isolates of the PRN serotype were tested, all the non-transmissible ones reacted with these two MAbs, but so also did some of those that are readily transmissible. SCR 80 and 81 were able to trap TRV-PLB particles onto electron microscope grids and reacted with metatopes located near the C terminus of the particle protein. The epitope recognized by SCR 81 was discontinuous. The MAbs that reacted with both TRV-PLB and TRV-PPK20 recognized either continuous cryptotopes (SCR 78) or discontinuous neotopes (SCR 79 and 82).

引用

页码：1497 / 1501

页数：5

共 16 条

[1] IMMUNOCHEMICAL STUDIES OF TOBACCO MOSAIC-VIRUS .6. ATTEMPTS TO LOCALIZE VIRAL EPITOPES WITH MONOCLONAL-ANTIBODIES [J].