NA-23-NUCLEAR MAGNETIC-RESONANCE INVESTIGATION OF GRAMICIDIN-INDUCED ION-TRANSPORT THROUGH MEMBRANES UNDER EQUILIBRIUM CONDITIONS

被引：59

作者：

BUSTER, DC

HINTON, JF

MILLETT, FS

SHUNGU, DC

机构：

[1] UNIV ARKANSAS, DEPT CHEM BIOCHEM, FAYETTEVILLE, AR 72701 USA

[2] PENN STATE UNIV, MILTON S HERSHEY MED CTR, DEPT RADIOL, HERSHEY, PA 17033 USA

来源：

BIOPHYSICAL JOURNAL | 1988年 / 53卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(88)83076-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A technique for investigating the gramicidin-facilitated transport of Na+ ions across lipid bilayers of large unilamellar vesicles under the condition of ionic equilibrium has been developed using a combination of heat incubation of the gramicidin with the vesicles and 23Na-nuclear magnetic resonance (NMR) spectroscopy. Isolation of the two 23Na-NMR signals from the intra- and extravesicular Na+ with the shift reagent, dysprosium (III) tripolyphosphate, allows the equilibrium flux of Na+ through the gramicidin channels to be detected and treated as a two-site exchange process. This study indicates that the transport of Na+ through gramicidin channels is second order with respect to the gramicidin concentration.

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页码：145 / 152

页数：8

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