SPIN-SPIN RELAXATION OF THE PHOSPHODIESTER RESONANCE IN THE P-31 NMR-SPECTRUM OF HUMAN BRAIN - THE DETERMINATION OF THE CONCENTRATIONS OF PHOSPHODIESTER COMPONENTS

被引：25

作者：

KILBY, PM ^{[1
]}

ALLIS, JL ^{[1
]}

RADDA, GK ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,MRC,BIOCHEM & CLIN MAGNET RESONANCE UNIT,S PARKS RD,OXFORD OX1 3QU,ENGLAND

来源：

FEBS LETTERS | 1990年 / 272卷 / 1-2期

关键词：

Human brain; Nuclear magnetic resonance; Spin-spin relaxation;

D O I：

10.1016/0014-5793(90)80474-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The phosphodiester peak in 31P nuclear magnetic resonance spectra of human brain in vivo is often the most prominent feature of the spectrum. We have demonstrated that this resonance exhibits bi-exponential spin-spin relaxation, giving relaxation times of 2 and 10 ms. We interpret this in terms of the two components which make up the peak, bilayer lipids and the small cytosolic phosphates glycerophosphoethanolamine and glycerophosphocholine. Using the relaxation times and the relative peak heights of the two components we have also been able to quantitate the concentration of the bilayer lipids as 20-40 times that of ATP. © 1990.

引用

页码：163 / 165

页数：3

共 9 条

[1]

ALLIS JL, 1990, IN PRESS J MAGN RESO

[2] QUANTITATIVE STUDIES OF HUMAN CARDIAC METABOLISM BY P-31 ROTATING-FRAME NMR [J].