IMMORTALIZATION OF HUMAN T-LYMPHOCYTES BY ONCOGENES

被引：3

作者：

OKANO, F ^{[1
]}

TACHIBANA, H ^{[1
]}

AKIYAMA, K ^{[1
]}

SHIRAHATA, S ^{[1
]}

MURAKAMI, H ^{[1
]}

机构：

[1] EHIME UNIV,DEPT AGR,MATSUYAMA,EHIME 790,JAPAN

来源：

CYTOTECHNOLOGY | 1993年 / 11卷 / 03期

关键词：

HUMAN T-LYMPHOCYTES; IMMORTALIZATION; ONCOGENES;

D O I：

10.1007/BF00749871

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2 (IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.

引用

页码：205 / 211

页数：7

共 16 条

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