NASAL CHALLENGE WITH ALLERGEN UP-REGULATES THE LOCAL EXPRESSION OF VASCULAR ENDOTHELIAL ADHESION MOLECULES

被引:59
作者
LEE, BJ
NACLERIO, RM
BOCHNER, BS
TAYLOR, RM
LIM, MC
BAROODY, FM
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MED,DIV CLIN IMMUNOL,BALTIMORE,MD 21218
[2] JOHNS HOPKINS UNIV,SCH MED,DEPT OTOLARYNGOL HEAD & NECK SURG,BALTIMORE,MD 21218
[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PATHOL,BALTIMORE,MD 21218
关键词
ADHESION MOLECULES; INTERCELLULAR ADHESION MOLECULE-1; E-SELECTIN; VASCULAR CELL ADHESION MOLECULE-1; ALLERGIC RHINITIS; NASAL BIOPSY; EOSINOPHIL;
D O I
10.1016/0091-6749(94)90119-8
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
To understand the events involved in selective eosinophil migration into allergic inflammatory sites, we studied the expression of vascular endothelial adhesion molecules in the nasal mucosa. Ten subjects with asymptomatic seasonal allergic rhinitis and 13 nonallergic subjects underwent localized allergen challenge of one inferior turbinate. Twenty-four hours later; biopsy specimens were obtained from the inferior turbinates, bilaterally in the seasonally allergic subjects and unilaterally in the nonallergic control subjects. The specimens were divided, sectioned, and either stained for identification of eosinophils or analyzed immunohistochemically for intercellularlar adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and von Willebrand's factor. Intercellular adhesion molecule-1 expression was observed in all mucosal specimens, with no significant difference among groups. E-selectin showed minimal baseline expression, and low levels were significantly induced on the challenged mucosa of the allergic compared with nonallergic subjects (p < 0.05). VCAM-2 was expressed basally and was significantly upregulated by allergen challenge compared with the nonchallenged side and nonallergic control subjects (p < 0.05). Submucosal eosinophils increased significantly in allergic subjects 24 hours after antigen challenge, compared with nonallergic control subjects and weakly correlated with VCAM-1 expression (r(s) = 0.33, p = 0.06). Our results suggest that endothelial activation accompanies allergic inflammation. Furthermore because the counter ligand for VCAM-1, very late activation antigen-4, is present on eosinophils, VCAM-1 upregulation may contribute to the selective recruitment of these cells to the nasal mucosa.
引用
收藏
页码:1006 / 1016
页数:11
相关论文
共 41 条
[1]  
ALTMAN L C, 1991, Journal of Allergy and Clinical Immunology, V87, P304, DOI 10.1016/0091-6749(91)91941-L
[2]  
BACHERT C, 1991, AM J RHINOL, V5, P227
[3]   THE INFLUX OF INFLAMMATORY CELLS INTO NASAL WASHINGS DURING THE LATE RESPONSE TO ANTIGEN CHALLENGE - EFFECT OF SYSTEMIC STEROID PRETREATMENT [J].
BASCOM, R ;
PIPKORN, U ;
LICHTENSTEIN, LM ;
NACLERIO, RM .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 138 (02) :406-412
[4]   IMMUNOHISTOLOGY OF THE NASAL-MUCOSA IN SEASONAL ALLERGIC RHINITIS - INCREASES IN ACTIVATED EOSINOPHILS AND EPITHELIAL MAST-CELLS [J].
BENTLEY, AM ;
JACOBSON, MR ;
CUMBERWORTH, V ;
BARKANS, JR ;
MOQBEL, R ;
SCHWARTZ, LB ;
IRANI, AMA ;
KAY, AB ;
DURHAM, SR .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (04) :877-883
[5]   ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS [J].
BEVILACQUA, MP ;
STENGELIN, S ;
GIMBRONE, MA ;
SEED, B .
SCIENCE, 1989, 243 (4895) :1160-1165
[6]   SELECTINS [J].
BEVILACQUA, MP ;
NELSON, RM .
JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (02) :379-387
[7]  
BIRDSALL HH, 1992, J IMMUNOL, V148, P2717
[8]  
BOCHNER BS, 1987, J IMMUNOL, V139, P2297
[9]   ADHESION OF HUMAN BASOPHILS, EOSINOPHILS, AND NEUTROPHILS TO INTERLEUKIN 1-ACTIVATED HUMAN VASCULAR ENDOTHELIAL-CELLS - CONTRIBUTIONS OF ENDOTHELIAL-CELL ADHESION MOLECULES [J].
BOCHNER, BS ;
LUSCINSKAS, FW ;
GIMBRONE, MA ;
NEWMAN, W ;
STERBINSKY, SA ;
DERSEANTHONY, CP ;
KLUNK, D ;
SCHLEIMER, RP .
JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 173 (06) :1553-1556
[10]  
BOCHNER BS, 1994, IN PRESS EOSINOPHILS