CRYOPRESERVATION OF RABBIT ZYGOTES

被引：8

作者：

ALHASANI, S

HEPNAR, C

DIEDRICH, K

VANDERVEN, H

KREBS, D

机构：

[1] UniversitSts-Frauenklinik und Hebammenlehranstalt, Bonn

来源：

HUMAN REPRODUCTION | 1992年 / 7卷

关键词：

D O I：

10.1093/humrep/7.suppl_1.81

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Rabbit embryos at the pronuclear stage (n = 480) were frozen using a mixture of propanediol and sucrose. The survival rate was 71% according to their morphological features shortly after thawing. The viability rate after 5 days of culture was 10% according to the number of embryos that reached the blastocyst stage, while the rate of formation of blastocysts from freshly collected and cultured pronuclear embryos was higher (59%). The implantation rate was higher (24%) if the frozen/thawed zygotes were directly transferred to foster mothers after 24 hours of culture, whereas with zygotes cultured for 24 hours (4 to 8-cell stages) the implantation rate was poor (4%). Both rates were low compared with the implantation rate (52%) achieved with freshly collected and directly transferred pronucleate embryos.

引用

页码：81 / 83

页数：3

共 7 条

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