UBIQUINONE BIOSYNTHESIS - CLONING OF THE GENES-CODING FOR CHORISMATE PYRUVATE-LYASE AND 4-HYDROXYBENZOATE OCTAPRENYL TRANSFERASE FROM ESCHERICHIA-COLI

被引：67

作者：

SIEBERT, M

BECHTHOLD, A

MELZER, M

MAY, U

BERGER, U

SCHRODER, G

SCHRODER, J

SEVERIN, K

HEIDE, L

机构：

[1] UNIV FREIBURG,INST PHARMAZEUT BIOL,SCHANZLESTR 1,W-7800 FREIBURG,GERMANY

[2] UNIV BONN,INST PHARMAZEUT BIOL,W-5300 BONN 1,GERMANY

[3] UNIV BONN,INST BIOL 2,W-5300 BONN 1,GERMANY

来源：

FEBS LETTERS | 1992年 / 307卷 / 03期

关键词：

UBIQUINONE; CHORISMATE PYRUVATE-LYASE; UBIC; 4-HYDROXYBENZOATE POLYPRENYL TRANSFERASE; UBIA; ESCHERICHIA-COLI;

D O I：

10.1016/0014-5793(92)80710-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chorismate pyruvate-lyase activity was detected in extracts of Escherichia coli. 4-Hydroxybenzoate was identified as the product of the enzymatic reaction by chemical derivatization and GC-MS analysis. The ubiC gene, coding for the chorismate pyruvate-lyase, was cloned and sequenced. The molecular weight of the gene product was calculated as 18,776 Da and confirmed by expression of the protein in E. coli minicells. The ubiA gene, coding for the 4-hydroxybenzoate octaprenyl transferase, was identified by sequence homology and complementation of a ubiA- strain. It is located directly downstream of ubiC in a typical operon structure.

引用

页码：347 / 350

页数：4

共 15 条

[1]

ASHBY MN, 1992, J BIOL CHEM, V267, P4128

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