INDUCTION OF CYP ISOENZYMES IN VARIOUS ORGANS OF RATS BY 3-METHYLCHOLANTHRENE OR BETA-NAPHTHOFLAVONE

被引：29

作者：

MATSUDA, T ^{[1
]}

IMAOKA, S ^{[1
]}

FUNAE, Y ^{[1
]}

OTORI, K ^{[1
]}

FUKUSHIMA, S ^{[1
]}

机构：

[1] OSAKA CITY UNIV,SCH MED,CHEM LAB,OSAKA 545,JAPAN

来源：

CANCER LETTERS | 1995年 / 97卷 / 02期

关键词：

CYTOCHROME P-450; 3-METHYLCHOLANTHRENE; B-NAPHTHOFLAVONE;

D O I：

10.1016/0304-3835(95)03965-Y

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Induction of cytochrome P450 (CYP) isoenzymes in various organs of rats treated with 3-methylcholanthrene (3-MC) and beta-naphthoflavone (BNF) was immunohistochemically and biochemically investigated. Fifteen male F344 rats were divided into three equal groups. Group 1 was untreated as a control. On days 3, 4, 13 and 14, group 2 animals received 3-MC (20 mg/kg body wt i.p.) dissolved in corn oil while group 3 animals were given BNF (50 mg/kg body wt i.p.) dissolved in corn oil, at days 1, 2, 3, 4, 11, 12, 13 and 14. On days 4 and 14, two or three animals in each group were sacrificed, 15 h after administration of the last test compound. Induction of CYP 1A1, 2C11, 2D1, 2E1, 3A2 and 4A1 in various organs was immunohistochemically examined and the levels of CYP 1A1 protein were measured by Western blotting. 3-MC and BNF induced CYP isoenzymes not only in the liver, but also in the small intestine, large intestine, prostate and seminal vesicles. The results indicate that xenobiotic metabolism can occur in various organs.

引用

页码：137 / 143

页数：7

共 29 条

[1] DOSE-DEPENDENT ACINAR INDUCTION OF CYTOCHROMES-P450 IN RAT-LIVER - EVIDENCE FOR A DIFFERENTIAL MECHANISM OF INDUCTION OF P450IA1 BY BETA-NAPHTHOFLAVONE AND DIOXIN [J].