STRUCTURAL STUDY OF CELL-WALL PHOSPHOMANNAN OF CANDIDA-ALBICANS NIH-B-792 (SEROTYPE-B) STRAIN, WITH SPECIAL REFERENCE TO H-1 AND C-13 NMR ANALYSES OF ACID-LABILE OLIGOMANNOSYL RESIDUES

Chemical structures of manno-oligosaccharides, from biose to heptaose, released from the phosphomannan of Candida albicans NIH B-792 strain (serotype B) by mild acid hydrolysis were investigated. The results of 1H NMR, 13C NMR, and fast atom bombardment mass spectrometry analyses confirmed that these manno-oli gosaccharides belong to a homologous β-1,2-linked series. Although chemical shifts of 1H NMR patterns of these oligosaccharides were considerably too complicated to be assigned, their 13C NMR patterns were sufficiently simple to be interpreted, exhibiting a regular increase of downfield shift of ppm values of the C-1 atom from each mannopyranose residue in proportion to their molecular weights. In order to determine the whole chemical structure of the parent phosphomannan, the acid-stable domain was subjected to acetolysis and then enzymolysis with the Arthrobacter GJM-1 α-mannosidase and the resultant manno-oligosaccharides were investigated for their chemical structures by 1H NMR spectroscopy. The results of a precipitin-inhibition test using the β-1,2-linked manno-oligosaccharides, from biose to hexaose, in comparison with the corresponding isomers containing α-1,2 linkage with small amounts of α-1,3 linkage, indicated that the haptens possessing the former linkage exhibited much higher inhibitory effects than the corresponding isomers containing the latter linkages did. Based on the present findings, a chemical structure of the phosphomannan of this C. albicans strain was proposed. © 1990.