QUANTIFICATION OF THE DETECTION OF PNEUMOCYSTIS-CARINII BY DNA AMPLIFICATION

被引:32
作者
PETERS, SE [1 ]
WAKEFIELD, AE [1 ]
BANERJI, S [1 ]
HOPKIN, JM [1 ]
机构
[1] CHURCHILL HOSP,OSLER CHEST UNIT,OXFORD OX3 7LJ,ENGLAND
基金
英国惠康基金;
关键词
PNEUMOCYSTIS-CARINII; DNA AMPLIFICATION; PCR; QUANTITATIVE DETECTION;
D O I
10.1016/0890-8508(92)90055-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a highly specific and sensitive technique for the detection of Pneumocystis carinii DNA using DNA amplification by the polymerase chain reaction (PCR). PCR products are detected by agarose gel electrophoresis and Southern hybridization to an oligonucleotide probe. Here we report the calibration of parasite numbers with amplification and hybridization signals and show that we can detect P. carinii to a lower limit of one to two organisms. The quantification of this diagnostic technique allows us to establish the number of organisms in a clinical sample which correspond to pneumocystis pneumonia or to sub-clinical pulmonary colonization. © 1992.
引用
收藏
页码:115 / 117
页数:3
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