ANALYSIS OF THE EXPRESSION AND SECRETION OF ISOTYPES OF SHEEP B-CELL IMMUNOGLOBULINS WITH A PANEL OF ISOTYPE-SPECIFIC MONOCLONAL-ANTIBODIES

被引：17

作者：

BIRD, P

JONES, P

ALLEN, D

DONACHIE, W

HUNTLEY, J

MCCONNELL, I

HOPKINS, J

机构：

[1] UNIV EDINBURGH,DEPT VET PATHOL,EDINBURGH EH9 1QH,MIDLOTHIAN,SCOTLAND

[2] MOREDUN RES INST,EDINBURGH EH17 7JH,MIDLOTHIAN,SCOTLAND

来源：

RESEARCH IN VETERINARY SCIENCE | 1995年 / 59卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0034-5288(95)90000-4

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Monoclonal antibodies to sheep light chain, IgM and IgG were produced and used to assess total immunoglobulin (Ig) synthesis by sheep B cells in culture and antibodies to specific antigens. By using these antibodies in a dual fluorescence-activated cell sorting analysis of sheep efferent lymph B lymphocytes the percentage change in surface Ig isotype of B lymphoblasts from IgM to IgG after the antigenic stimulation of the local lymph node was measured. An extension of this analysis to paired blood and afferent or efferent lymph B cells made it possible to investigate the recirculation characteristics of B cells expressing different Ig isotypes.

引用

页码：189 / 194

页数：6

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