MEASUREMENT OF XANTHOXIN IN HIGHER-PLANT TISSUES USING C-13 LABELED INTERNAL STANDARDS

被引：30

作者：

PARRY, AD ^{[1
]}

NEILL, SJ ^{[1
]}

HORGAN, R ^{[1
]}

机构：

[1] BRISTOL POLYTECH, DEPT SCI, BRISTOL BS16 1QY, ENGLAND

来源：

PHYTOCHEMISTRY | 1990年 / 29卷 / 04期

关键词：

2-trans-xanthoxin; !sup]13[!/sup]C-labelled internal standards; Compositae; GC-MS selected ion monitoring; Gramineae; Helianthus annuus; isomerization; Leguminosae; Lycopersicon esculentum; Phaseolus vulgaris; Solanaceae; xanthoxin; Zea mays;

D O I：

10.1016/0031-9422(90)85398-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new procedure for the reliable measurement of xanthoxin from plant tissues has been developed. This accounts for both its breakdown and its isomerization to 2-trans-xanthoxin, and relies on the use of 12C-labelled internal standards, HPLC purification and analysis by GC-MS SIM. The antioxidant tert-butylated hydroxyquinoline was found to reduce the amount of isomerization to 2-trans-xanthoxin during extraction. Levels of xanthoxin present in all tissues examined were much lower than previously thought, and 2-trans-xanthoxin: xanthoxin ratios much higher. © 1990.

引用

页码：1033 / 1039

页数：7

共 37 条

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