THE FLAVIVIRUS ENVELOPE PROTEIN-E - ISOLATION OF A SOLUBLE FORM FROM TICK-BORNE ENCEPHALITIS-VIRUS AND ITS CRYSTALLIZATION

被引：55

作者：

HEINZ, FX ^{[1
]}

MANDL, CW ^{[1
]}

HOLZMANN, H ^{[1
]}

KUNZ, C ^{[1
]}

HARRIS, BA ^{[1
]}

REY, F ^{[1
]}

HARRISON, SC ^{[1
]}

机构：

[1] HARVARD UNIV,HOWARD HUGHES MED INST,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 10期

关键词：

D O I：

10.1128/JVI.65.10.5579-5583.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By the use of limited trypsin digestion of purified virions, we generated a membrane anchor-free and crystallizable form of the tick-borne encephalitis virus envelope glycoprotein E. It retained its reactivity with a panel of monoclonal antibodies, and only subtle structural differences from the native protein E were recognized. Treatment with the bifunctional cross-linker dimethylsuberimidate resulted in the formation of a dimer. Crystallization experiments yielded hexagonal rod-shaped crystals suitable for X-ray diffraction analysis.

引用

页码：5579 / 5583

页数：5

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