IDENTIFICATION OF METABOLIC PATHWAYS OF THE LIPID-PEROXIDATION PRODUCT 4-HYDROXYNONENAL BY MITOCHONDRIA ISOLATED FROM RAT-KIDNEY CORTEX

被引：52

作者：

ULLRICH, O

GRUNE, T

HENKE, W

ESTERBAUER, H

SIEMS, WG

机构：

[1] HUMBOLDT UNIV BERLIN,FAC MED CHARITE,PHYS THERAPY CLIN,D-10098 BERLIN,GERMANY

[2] HUMBOLDT UNIV BERLIN,FAC MED CHARITE,REHABIL CLIN,D-10098 BERLIN,GERMANY

[3] HUMBOLDT UNIV BERLIN,FAC MED CHARITE,UROL CHAIR,DIV RES,D-10098 BERLIN,GERMANY

[4] KARL FRANZENS UNIV GRAZ,INST BIOCHEM,A-8010 GRAZ,AUSTRIA

[5] HERZOG JULIUS HOSP RHEUMATOL & ORTHOPAED,D-38655 BAD HARZBURG,GERMANY

来源：

FEBS LETTERS | 1994年 / 352卷 / 01期

关键词：

MITOCHONDRIA; 4-HYDROXYNONENAL; ALDEHYDE METABOLISM; 4-HYDROXYNONENOIC ACID; 1,4-DIHYDROXYNONENE; HNE-GLUTATHIONE;

D O I：

10.1016/0014-5793(94)00922-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glutathione-HNE-conjugate. Furthermore, formation of metabolites of the tricarboxylic acid cycle from HNE is suggested. The quantitative share of HNE binding to proteins was high with about 8% of total HNE consumption after 3 min of incubation. Therefore, rapid degradation of HNE by mitochondria might be involved in an intracellular antioxidative defense system.

引用

页码：84 / 86

页数：3

共 17 条

[1] INTERACTION BETWEEN NEUTROPHILS AND 4-HYDROXYALKENALS AND CONSEQUENCES ON NEUTROPHIL MOTILITY [J].