INHIBITION OF MAMMALIAN HEPATIC GLUTATHIONE S-TRANSFERASES BY ACETYLENIC FATTY-ACIDS

被引：6

作者：

DATTA, K ^{[1
]}

KULKARNI, AP ^{[1
]}

机构：

[1] UNIV S FLORIDA,COLL PUBL HLTH,FLORIDA TOXICOL RES CTR,TAMPA,FL 33612

来源：

TOXICOLOGY LETTERS | 1994年 / 73卷 / 02期

关键词：

GLUTATHIONE S-TRANSFERASE; INHIBITION; LIPOXYGENASE INHIBITORS; ACETYLENIC FATTY ACIDS;

D O I：

10.1016/0378-4274(94)90105-8

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Micromolar concentrations of 2 classical inhibitors of lipoxygenase, 5,8,11 -eicosatriynoic acid (ETI) and 5,8,11,14-eicosatetraynoic acid (ETYA), were found to cause a significant inhibition of the mixture of isozymes of affinity purifed rat and human liver glutathione S-transferase (GST) with activity towards 1-chloro-2,4-dinitrobenzene (CDNB). ETI was a more potent inhibitor of both rat and human liver GST than ETYA. Analysis of kinetic data suggested noncompetitive inhibition of human liver GST by ETI towards reduced glutathione and CDNB. ETI also inhibited the hepatic GST activity towards 1,2-dichloro-4-nitrobenzene, p-nitrobenzyl chloride and 4-nitropyridine N-oxide.

引用

页码：157 / 165

页数：9

共 34 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] MODULATION OF RESISTANCE TO ALKYLATING-AGENTS IN CANCER CELL BY GOSSYPOL ENANTIOMERS [J].

FORD, JM ;

HAIT, WN ;

MATLIN, SA ;

BENZ, CC .

CANCER LETTERS, 1991, 56 (01) :85-94

[3]

HABIG WH, 1974, J BIOL CHEM, V249, P7130

[4] GOSSYPOL, A POTENT INHIBITOR OF ARACHIDONATE 5-LIPOXYGENASE AND 12-LIPOXYGENASE [J].

HAMASAKI, Y ;

TAI, HH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 834 (01) :37-41

[5]

JAKSCHIK BA, 1982, ADV PROSTAG THROMB L, V9, P127

[6] METABOLISM OF THE NEPHROTOXIN DICHLOROACETYLENE BY GLUTATHIONE CONJUGATION [J].

KANHAI, W ;

DEKANT, W ;

HENSCHLER, D .

CHEMICAL RESEARCH IN TOXICOLOGY, 1989, 2 (01) :51-56

[7] ADJUVANT EFFECTS OF ANTINEOPLASTIC PROSTAGLANDINS TO CISPLATIN IN NUDE-MICE BEARING HUMAN OVARIAN-CANCER CELLS [J].

KIKUCHI, Y ;

KITA, T ;

MIYAUCHI, M ;

HIRATA, J ;

SASA, H ;

NAGATA, I ;

FUKUSHIMA, M .

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 1992, 118 (06) :453-457

[8] THE MECHANISM OF INACTIVATION OF LIPOXYGENASES BY ACETYLENIC FATTY-ACIDS [J].

KUHN, H ;

HOLZHUTTER, HG ;

SCHEWE, T ;

HIEBSCH, C ;

RAPOPORT, SM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (03) :577-583

[9] EVIDENCE FOR IDENTIFYING FATTY-ACIDS IN RAT-LIVER GLUTATHIONE-S-TRANSFERASE AND ITS POSSIBLE INVOLVEMENT IN THE SECONDARY STRUCTURE [J].

LI, M ;

ISHIBASHI, T .

JOURNAL OF BIOCHEMISTRY, 1990, 108 (03) :462-465

[10] GLUTATHIONE TRANSFERASES - STRUCTURE AND CATALYTIC ACTIVITY [J].

MANNERVIK, B ;

DANIELSON, UH .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1988, 23 (03) :283-337

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