CLONING AND EXPRESSION OF A HUMAN GLUTAMATE TRANSPORTER

被引：51

作者：

KAWAKAMI, H ^{[1
]}

TANAKA, K ^{[1
]}

NAKAYAMA, T ^{[1
]}

INOUE, K ^{[1
]}

NAKAMURA, S ^{[1
]}

机构：

[1] NCNP, NATL INST NEUROSCI, DEPT DEGENERAT NEUROL DIS, KODAIRA, TOKYO 187, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 199卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.1210

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complementary DNA clone was isolated from human brain that encodes a glutamate transporter. Injection of RNA transcribed from this cDNA into Xenopus oocytes resulted in expression of a transport activity with a high affinity for glutamate (Km = 78.4 mu M) and a dependency on external Na+. The cDNA sequence predicts a protein of 542 amino acids that is highly homologous to the congeneric proteins from rat and mouse brains. RNA blotting analyses revealed the expression both in the brain and in peripheral tissues. (C) 1994 Academic Press, Inc.

引用

页码：171 / 176

页数：6

共 21 条

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