ADHERENCE TO EXPERIMENTAL PELLICLE OF ROUGH-TYPE LIPOPOLYSACCHARIDES FROM SUBGINGIVAL PLAQUE BACTERIA

被引：14

作者：

OKUDA, K

KATO, T

ISHIHARA, K

NAITO, Y

机构：

[1] Department of Microbiology, Tokyo Dental College, Chiba City, Masago

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 04期

关键词：

LIPOPOLYSACCHARIDE; SUBGINGIVAL PLAQUE BACTERIA; ADHERENT ACTIVITY; EXPERIMENTAL PELLICLE;

D O I：

10.1111/j.1399-302X.1991.tb00484.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The adherence of lipopolysaccharides (LPSs) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (S-HA) and serum-coated HA beads was examined by chromogenic Limulus activity (toxicolor test). Phenol-water extracted LPS preparations from Bacteroides intermedius, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, Eikenella corrodens, and rough-type LPS from Escherichia coli adhered to S-HA and serum-coated beads and agglutinated human erythrocytes. The adhered LPSs to S-HA and serum-coated HA beads were not removed by vigorous washing with distilled water. LPSs from Bacteroides (Porphyromonas) gingivalis strains and wild-type E. coli did not adhere to S-HA, serum-coated HA beads or show hemagglutinating activity. SDS-PAGE patterns stained with silver stain showed that LPSs adhered to S-HA, and serum-coated HA beads and erythrocytes possessed a distinct fast-migrating band similar to rough-type LPS. B. gingivalis LPSs possessed slow-migrating and repeating ladder bands similar to wild-type LPS.

引用

页码：241 / 245

页数：5

共 29 条

[1]

Aleo JJ, de Renzis FA, Farber PA, Et al., The presence and biological activity of cementum‐bound endotoxin, J Periodontol, 45, pp. 672-675, (1974)

[2]

Aleo JJ, DeRenzis FA, Farber PA, In vitro attachment of human gingival fibroblasts to root surfaces, J Periodontol, 46, pp. 639-645, (1974)

[3]

Boyd J, McBride BC, Fractionation of hemagglutinating and bacterial binding adhesions of Bacteroides gingivalis, Infect Immun, 45, pp. 403-409, (1984)

[4]

Bramanti TE, Wong GG, Weintraub ST, Holt HC, Chemical characterization and biological properties of lipopolysaccharide from Bacteroides gingivalis strain W50, W83 and ATCC 33277, Oral Microbiol Immunol, 4, pp. 183-192, (1989)

[5]

Clark WB, Bammann LL, Gibbons RJ, Comparative estimates of bacterial affinities and adsorption sites to hydroxyapatite surfaces, Infect Immun, 19, pp. 846-853, (1978)

[6]

Darveau RP, Hancock REW, Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains, J Bacteriol, 155, pp. 831-838, (1983)

[7]

Gibbons RJ, van Houte J, Bacterial adherence in oral microbial ecology, Ann Rev Microbial, 29, pp. 19-44, (1975)

[8]

Hamada S, Koga T, Nishihara T, Et al., Characterization and immunological activities of lipopolysaccharides from periodontal bacteria, Adv Dent Res, 2, pp. 284-291, (1988)

[9]

Hughes FJ, Smales FC, Immunohistochemical investigation of the presence and distribution of cementum‐associated lipopolysaccharides in periodontal disease, J Periodont Res, 21, pp. 660-667, (1986)

[10]

Jones WA, O'Leary TJ, The effectiveness of in vivo root planing in removing bacterial endotoxin from the roots of periodontally involved teeth, J Periodontol, 49, pp. 337-342, (1978)

← 1 2 3 →