SEX ATTRACTANT PHEROMONE OF DAMSON-HOP APHID PHORODON-HUMULI (HOMOPTERA, APHIDIDAE)

被引:77
作者
CAMPBELL, CAM
DAWSON, GW
GRIFFITHS, DC
PETTERSSON, J
PICKETT, JA
WADHAMS, LJ
WOODCOCK, CM
机构
[1] AFRC,INST ARABLE CROPS RES,ROTHAMSTED EXPTL STN,HARPENDEN AL5 2JQ,HERTS,ENGLAND
[2] AFRC,INST HORT RES,MAIDSTONE ME19 6BJ,KENT,ENGLAND
[3] SWEDISH UNIV AGR SCI,S-75007 UPPSALA,SWEDEN
关键词
PHEROMONE; SEX; ATTRACTANT; APHID; PHORODON-HUMULI; HOMOPTERA; APHIDIDAE; NEPETALACTOL; MONOTERPENOID; ELECTROPHYSIOLOGY; SINGLE CELL RECORDING; MASS SPECTROMETRY;
D O I
10.1007/BF00982110
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Behavioral studies using an olfactometer demonstrated that sexual females (oviparae) of the damson-hop aphid, Phorodon humuli, release a pheromone to which males respond. Volatiles produced by the oviparae were analyzed by coupled gas chromatography-single cell recording from the secondary rhinaria on the male antenna and showed the presence of one peak with major activity. Coupled gas chromatography-mass spectrometry suggested a nepetalactol, which was shown to have the 4aR, 7S, 7aS stereo-chemistry by synthesis from the corresponding nepetalactone isolated from the labiate plant Nepeta mussinii. Although the stereochemistry at carbon-1 is not yet established, a synthetic sample comprising ca. 70% 1S and 30% 1R attracted highly significant numbers of males to water traps placed within and adjacent to a hop garden. Initial studies also indicated attraction of males in both the olfactometer and in the field by volatiles from the primary host.
引用
收藏
页码:3455 / 3465
页数:11
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