ISOLATION AND FUNCTIONAL EXPRESSION OF PITUITARY PEPTIDYLGLYCINE ALPHA-AMIDATING ENZYME MESSENGER-RNA - A VARIANT LACKING THE TRANSMEMBRANE DOMAIN

被引：26

作者：

KATO, I ^{[1
]}

YONEKURA, H ^{[1
]}

YAMAMOTO, H ^{[1
]}

OKAMOTO, H ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT BIOCHEM,SENDAI,MIYAGI 980,JAPAN

来源：

FEBS LETTERS | 1990年 / 269卷 / 02期

关键词：

Alternative splicing; Functional expression; mRNA; Peptide α-amidating enzyme; Secretory form; Transmembrane domain;

D O I：

10.1016/0014-5793(90)81184-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme. © 1990.

引用

页码：319 / 323

页数：5

共 19 条

[1] MECHANISM OF C-TERMINAL AMIDE FORMATION BY PITUITARY ENZYMES [J].

BRADBURY, AF ;

FINNIE, MDA ;

SMYTH, DG .

NATURE, 1982, 298 (5875) :686-688

[2] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[3] STRUCTURE OF THE PRECURSOR TO AN ENZYME MEDIATING COOH-TERMINAL AMIDATION IN PEPTIDE BIOSYNTHESIS [J].

EIPPER, BA ;

PARK, LP ;

DICKERSON, IM ;

KEUTMANN, HT ;

THIELE, EA ;

RODRIGUEZ, H ;

SCHOFIELD, PR ;

MAINS, RE .

MOLECULAR ENDOCRINOLOGY, 1987, 1 (11) :777-790

[4]

EIPPER BA, 1988, J BIOL CHEM, V263, P8371

[5] PEPTIDYL-GLYCINE ALPHA-AMIDATION ACTIVITY IN TISSUES AND SERUM OF THE ADULT-RAT [J].

EIPPER, BA ;

MYERS, AC ;

MAINS, RE .

ENDOCRINOLOGY, 1985, 116 (06) :2497-2504

[6] EVOLUTIONARY CONSERVATION OF THE INSULINOMA GENE RIG AND ITS POSSIBLE FUNCTION [J].

INOUE, C ;

SHIGA, K ;

TAKASAWA, S ;

KITAGAWA, M ;

YAMAMOTO, H ;

OKAMOTO, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6659-6662

[7] CDNA HETEROGENEITY SUGGESTS STRUCTURAL VARIANTS RELATED TO THE HIGH-AFFINITY IGE RECEPTOR [J].

LIU, FT ;

ALBRANDT, K ;

ROBERTSON, MW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5639-5643

[8]

MAY V, 1988, J BIOL CHEM, V263, P7550

[9] PURIFICATION OF A PEPTIDYLGLYCINE ALPHA-AMIDATING ENZYME FROM TRANSPLANTABLE RAT MEDULLARY-THYROID CARCINOMAS [J].

MEHTA, NM ;

GILLIGAN, JP ;

JONES, BN ;

BERTELSEN, AH ;

ROOS, BA ;

BIRNBAUM, RS .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 261 (01) :44-54

[10]

MESSING J, 1983, METHOD ENZYMOL, V101, P20

← 1 2 →