CLONING AND EXPRESSION OF A GLUTAMATE TRANSPORTER FROM MOUSE-BRAIN

被引：71

作者：

TANAKA, K

机构：

[1] Laboratory for Neural Networks, Frontier Research Program, The Institute of Physical, Wako, Saitama

来源：

NEUROSCIENCE LETTERS | 1993年 / 159卷 / 1-2期

关键词：

MOLECULAR CLONING; GLUTAMATE TRANSPORTER; MOUSE BRAIN; XENOPUS OOCYTE;

D O I：

10.1016/0304-3940(93)90829-A

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A complementary DNA clone encoding a transporter for glutamate has been isolated from mouse brain, and its functional properties have been examined in Xenopus oocytes. The transporter displays high affinity for glutamate (K(m) = 72 muM) and is dependent on external Na+. The cDNA sequence predicts a protein of 543 amino acids with 6 potential transmembrane domains. Northern blot analysis indicates the mRNA encoding the glutamate transporter is expressed both in the brain and in peripheral tissues.

引用

页码：183 / 186

页数：4

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