A PREPARATIVE METHOD FOR THE ISOLATION OF THE 43-KDA, 47-KDA AND THE D1-D2-CYT-B559 SPECIES DIRECTLY FROM THYLAKOID MEMBRANES

被引：13

作者：

FOTINOU, C

GHANOTAKIS, DF

机构：

[1] Department of Chemistry, University of Crete, Crete, Iraklion

来源：

PHOTOSYNTHESIS RESEARCH | 1990年 / 25卷 / 02期

关键词：

photochemistry; photosystem II; polypeptides; reaction center;

D O I：

10.1007/BF00035463

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Using the non-ionic detergent dodecyl-β-D-maltoside we have developed a preparative method for the isolation of the 43 kDa, 47 kDa and D1-D2-Cyt b559 species directly from thylakoid membranes. In contrast to previous procedures the photosynthetic membrane was exposed only to one mild detergent and that resulted in more stable preparations. The isolated species were examined spectroscopically and it was found that even under these mild conditions the D1-D2-Cyt b559 did not retain the primary quinone QA. © 1990 Kluwer Academic Publishers.

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页码：141 / 145

页数：5

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