CHARACTERIZATION AND GENERATION OF ESCHERICHIA-COLI ADENYLATE-CYCLASE DELETION MUTANTS

被引：45

作者：

SHAH, S ^{[1
]}

PETERKOFSKY, A ^{[1
]}

机构：

[1] NHLBI,BIOCHEM GENET LAB,BETHESDA,MD 20892

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 10期

关键词：

D O I：

10.1128/JB.173.10.3238-3242.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Escherichia coli DELTA-cya-283 is a 75-bp in-frame deletion overlapping the 5' end of DELTA-cya-854; DELTA-cya-201 is a 41-bp frameshift deletion overlapping the 3' end of DELTA-cya-854. Sequence repeats were found at the boundaries of DELTA-cya-283 and DELTA-cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene.

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页码：3238 / 3242

页数：5

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