PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF TRYPANOTHIONE REDUCTASE FROM CRITHIDIA-FASCICULATA

被引：15

作者：

KURIYAN, J ^{[1
]}

WONG, L ^{[1
]}

GUENTHER, BD ^{[1
]}

MURGOLO, NJ ^{[1
]}

CERAMI, A ^{[1
]}

HENDERSON, GB ^{[1
]}

机构：

[1] ROCKEFELLER UNIV,MED BIOCHEM LAB,NEW YORK,NY 10021

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 215卷 / 03期

关键词：

D O I：

10.1016/S0022-2836(05)80353-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trypanothione reductase, a flavoprotein disulfide reductase specific to trypanosomatid parasites, has been crystallized by vapor diffusion of a protein solution (10 mg/ml) against 22% polyethylene glycol (average Mr 8000) containing 100 mm-ammonium sulfate. Crystals of a size suitable for structure determination by X-ray diffraction have been obtained by seeding protein solutions with smaller crystals. The space-group is P21 (a=60·9 , b=161·8 , c=58·4 , β=99·10). The molecular mass and volume of the unit cell suggest that there is a dimer of the enzyme in the asymmetric unit, and this is confirmed by self-rotation functions calculated using data to 4·5resolution. The crystals diffract to beyond 3resolution. Crystals of another P21 form (a=91·3 , b=114·4 , c=92·0β=141·30) are observed to grow under similar conditions. © 1990, Academic Press Limited. All rights reserved.

引用

页码：335 / 337

页数：3

共 20 条

[1]

Crowther R. A., 1972, MOL REPLACEMENT METH, P173

[2] TRYPANOTHIONE - A NOVEL BIS(GLUTATHIONYL)SPERMIDINE COFACTOR FOR GLUTATHIONE-REDUCTASE IN TRYPANOSOMATIDS [J].