MODELING THE NON-STEADY-STATE RESPONSE OF AN ENZYME ELECTRODE FOR LACTOSE

被引：7

作者：

GARCIA, JL ^{[1
]}

LOPEZMUNGUIA, A ^{[1
]}

GALINDO, E ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MEXICO,CTR INVEST SOBRE INGN GENET & BIOTECNOL,DEPT BIOINGN,AP 5103,CUERNAVACA 62271,MEXICO

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 08期

关键词：

MODELING; ENZYME ELECTRODE; LACTOSE;

D O I：

10.1016/0141-0229(91)90083-M

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An amperometric enzyme electrode for lactose was developed by entrapment of glucose oxidase and beta-galactosidase. The electrode response was described by Fick's second law of diffusion, solved numerically by an algorithm based on the Schmidt plot graphical method. With this model, the effective diffusivity calculated for lactose was 1.1 x 10(-8) cm2 s-1. This simplified solution predicted accurately the experimental results and could be used for the estimation of effective diffusivities in the electrode membranes.

引用

页码：672 / 675

页数：4

共 18 条

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