CYTOSINE METHYLATION ENHANCES MITOMYCIN-C CROSS-LINKING

被引：26

作者：

MILLARD, JT

BEACHY, TM

机构：

[1] Department of Chemistry, Colby College, Waterville

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 47期

关键词：

D O I：

10.1021/bi00210a038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitomycin C (MC) is a powerful antitumor agent that targets the DNA sequence CpG. Because it is likely that this dinucleotide will contain 5-methylcytosine in vivo, we have compared the cross-linking efficiency of MC for DNA containing either 5-methylcytosine or normal cytosine embedded in random-sequence DNA oligomers. We found that mitomycin C displays a small but significant preference for methylated DNA. Recognition of an abnormal methylation pattern in the DNA of transformed cells may therefore be one mechanism by which MC exerts its chemotherapeutic effects.

引用

页码：12850 / 12856

页数：7

共 37 条

[1] HIGH-LEVELS OF DENOVO METHYLATION AND ALTERED CHROMATIN STRUCTURE AT CPG ISLANDS IN CELL-LINES [J].