EFFECT OF OKADAIC ACID ON PROTEIN-PHOSPHORYLATION PATTERNS OF CHICKEN MYOGENIC CELLS WITH SPECIAL REFERENCE TO CREATINE-KINASE

被引：21

作者：

HEMMER, W ^{[1
]}

SKARLI, M ^{[1
]}

PERRIARD, JC ^{[1
]}

WALLIMANN, T ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, INST CELL BIOL, CH-8093 ZURICH, SWITZERLAND

来源：

FEBS LETTERS | 1993年 / 327卷 / 01期

关键词：

PHOSPHORYLATION OF CREATINE KINASE; OKADAIC ACID; CHICKEN; MYOGENIC CELL;

D O I：

10.1016/0014-5793(93)81034-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Okadaic acid and other agents affecting cellular phosphorylation and dephosphorylation processes profoundly changed the phosphoprotein pattern of P-32i-labelled chicken embryonic skeletal muscle cells. The phosphorylation states of proteins in the lower molecular weight range were especially increased. Immunoprecipitation of cellular extracts with anti-creatine kinase antibodies enabled us to identify creatine kinase (CK) phosphoproteins. B-CK was phosphorylated after treating the cultures with 1-oleoyl-2-acetyl-sn-glycerol, dibutyryl-cAMP, okadaic acid and combinations thereof, but not with 1,2-dioleoyl-sn-glycerol. M-CK was also shown to be phosphorylated. The results indicated that in vivo, CK isoforms in muscle are subjected to control mediated by phosphorylation and dephosphorylation processes.

引用

页码：35 / 40

页数：6

共 26 条

[1] INHIBITORY EFFECT OF A MARINE-SPONGE TOXIN, OKADAIC ACID, ON PROTEIN PHOSPHATASES - SPECIFICITY AND KINETICS [J].

BIALOJAN, C ;

TAKAI, A .

BIOCHEMICAL JOURNAL, 1988, 256 (01) :283-290

[2] REGULATION OF CREATINE PHOSPHOKINASE-B ACTIVITY BY PROTEIN-KINASE-C [J].

CHIDA, K ;

TSUNENAGA, M ;

KASAHARA, K ;

KOHNO, Y ;

KUROKI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) :346-350

[3] PURIFICATION AND IDENTIFICATION OF CREATINE PHOSPHOKINASE-B AS A SUBSTRATE OF PROTEIN-KINASE-C IN MOUSE SKIN INVIVO [J].

CHIDA, K ;

KASAHARA, K ;

TSUNENAGA, M ;

KOHNO, Y ;

YAMADA, S ;

OHMI, S ;

KUROKI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) :351-357

[4] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].

COHEN, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508

[5] ROLE OF PROTEIN KINASE-C IN CHICK-EMBRYO SKELETAL MYOBLAST FUSION [J].

DAVID, JD ;

FASER, CR ;

PERROT, GP .

DEVELOPMENTAL BIOLOGY, 1990, 139 (01) :89-99

[6] MYOBLAST FUSION IS REGULATED BY A PROSTANOID OF THE ONE SERIES INDEPENDENTLY OF A RISE IN CYCLIC-AMP [J].

ENTWISTLE, A ;

CURTIS, DH ;

ZALIN, RJ .

JOURNAL OF CELL BIOLOGY, 1986, 103 (03) :857-866

[7]

FRIEDMAN DL, 1990, FASEB J, V4, pA2232

[8] REGULATORY INTERACTIONS OF CALMODULIN-BINDING PROTEINS - PHOSPHORYLATION OF CALCINEURIN BY AUTOPHOSPHORYLATED CA-2+ CALMODULIN-DEPENDENT PROTEIN KINASE-II [J].

HASHIMOTO, Y ;

KING, MM ;

SODERLING, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :7001-7005

[9] EFFECTS OF THE TUMOR PROMOTER OKADAIC ACID ON INTRACELLULAR PROTEIN-PHOSPHORYLATION AND METABOLISM [J].

HAYSTEAD, TAJ ;

SIM, ATR ;

CARLING, D ;

HONNOR, RC ;

TSUKITANI, Y ;

COHEN, P ;

HARDIE, DG .

NATURE, 1989, 337 (6202) :78-81

[10]

HEMMER W, 1993, THESIS SWISS FEDERAL

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