GINKGO-BILOBA EXTRACT (EGB-761) PROTECTS HUMAN LOW-DENSITY LIPOPROTEINS AGAINST OXIDATIVE MODIFICATION MEDIATED BY COPPER

The antioxidant effects of Ginkgo biloba extract (EGb 761) on copper-mediated human low density lipoprotein (LDL) oxidative modification were evaluated by several techniques. Human LDL (0.5 mg/ml) in phosphate buffered saline, pH 7.4, was incubated with 10 mu M cupric sulfate at 37 degrees C under air for 8 hours and 24 hours in the presence of varying concentrations of EGb 761. Increases in LDL apoB carbonylation, lipid peroxidation, apoB electrophoretic mobility and LDL fluorescence were all inhibited when the incubation mixture contained EGb 761 at concentrations less than 100 mu g/ml. This inhibition was EGb 761-concentration-dependent. Thus, EGb 761 has powerful antioxidant effects on cooper-mediated LDL oxidative modification. (C) 1995 Academic Press, Inc.