DETERMINATION OF PIRIBEDIL AND ITS BASIC METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：25

作者：

SARATI, S ^{[1
]}

GUISO, G ^{[1
]}

SPINELLI, R ^{[1
]}

CACCIA, S ^{[1
]}

机构：

[1] MARIO NEGRI INST PHARMACOL RES,VIA ERITREA 64,I-20157 MILAN,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 563卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80038-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of piribedil and its p-hydroxylated, catechol and N-oxide metabolites in plasma is described. After addition of an internal standard (buspirone), the plasma samples were subjected to a three-step extraction procedure. The final extracts were evaporated to dryness under nitrogen, and the residues were reconstituted in 100-mu-l of mobile phase (0.01 M phosphate buffer-acetonitrile, 50:50, v/v) and chromatographed by acetonitrile gradient elution on a C18 reversed-phase column coupled to an ultraviolet detector set at 240 nm. The method was selective for piribedil and its metabolites, and sufficiently sensitive and precise for studies aimed at elucidating the role of the metabolites in the parent drug's pharmacological effects.

引用

页码：323 / 332

页数：10

共 13 条

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