DIRECT SERUM INJECTION IN ION CHROMATOGRAPHY ON PACKING MATERIALS WITH A SEMIPERMEABLE SURFACE

被引：9

作者：

BUCHBERGER, W

MALISSA, H

MULLEDER, E

机构：

[1] Department of Analytical Chemistry, Johannes-Kepler-University

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 602卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80062-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Reversed-phase packing materials with restricted access of proteins to the hydrophobic sites were tested for their applicability in the ion-interaction chromatography of anions. Especially C-8-modified silica, which had been coated with a hydrophilic polymer acting as a semi-permeable barrier, could be used successfully for the separation of several anions in a proteinaceous matrix without removing the proteins prior to injection. In combination with UV or conductivity detection, this technique allows the determination of some physiologically important anions in serum samples.

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页码：51 / 55

页数：5

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