THE PREPARATION OF BIOTINYL-EPSILON-AMINOCAPROYLATED FORMS OF THE VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) AS PROBES FOR THE VIP RECEPTOR

被引：11

作者：

ANDERSSON, M

MARIE, JC

CARLQUIST, M

MUTT, V

机构：

[1] HOP ST ANTOINE, INSERM, U55, F-75571 PARIS 12, FRANCE

[2] KARO BIO AB, S-14104 HUDDINGE, SWEDEN

来源：

FEBS LETTERS | 1991年 / 282卷 / 01期

关键词：

VASOACTIVE INTESTINAL POLYPEPTIDE; PLASMA DESORPTION MASS SPECTROMETRY; BIOTINYLATED ANALOG; PIG LIVER MEMBRANE;

D O I：

10.1016/0014-5793(91)80439-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vasoactive intestinal polypeptide (VIP) was biotinyl-epsilon-aminocaproylated using sulfosuccinimidyl-6-(biotinamido) hexanoate thereby producing a series of products that were separated by high performance liquid chromatography (HPLC). Seven VIP-derivatives were isolated and the number and location of biotinyl-epsilon-aminocaproylation was determined by a combination of enzymatic degradation and plasma desorption mass spectrometry (PDMS). Receptor binding experiments with the VIP biotinyl-epsilon-aminocaproylated derivatives revealed IC50 values for the monobiotinyl-epsilon-aminocaproylated peptides that were 1.3-3.2 times higher than for natural VIP. All isolated biotinyl-epsilon-aminocaproylated derivatives possess VIP-like bioactivity as shown by an assay measuring pancreatic juice secretion in cat, VIP biotinyl-epsilon-aminocaproylated in position lysine 15 being almost equipotent with natural VIP.

引用

页码：35 / 40

页数：6

共 28 条

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