HUMAN PROTEASOME SUBUNITS FROM 2-DIMENSIONAL GELS IDENTIFIED BY PARTIAL SEQUENCING

被引：62

作者：

KRISTENSEN, P

JOHNSEN, AH

UERKVITZ, W

TANAKA, K

HENDIL, KB

机构：

[1] NATL UNIV HOSP,DEPT CLIN CHEM,COPENHAGEN,DENMARK

[2] UNIV TOKUSHIMA,INST ENZYME RES,TOKUSHIMA 770,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.2876

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human proteasomes consist of 14 major subunits which can be resolved by two-dimensional polyacrylamide gel electrophoresis. Tryptic peptides from the subunits were sequenced. This allowed identification of all proteins in two-dimensional electrophoresis gels with proteasome subunits whose sequences are known from cDNA. The results also precisely define the specificity of a panel of subunit-specific monoclonal antibodies. A new proteasome subunit (Z) was discovered. In contrast, the putative subunit MECL-1 could not be detected in human placenta proteasomes. (C) 1994 Academic Press, Inc.

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页码：1785 / 1789

页数：5

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