IMPROVED MEASUREMENT OF C-13, P-31 J-COUPLING-CONSTANTS IN ISOTOPICALLY LABELED RNA

被引：48

作者：

LEGAULT, P ^{[1
]}

JUCKER, FM ^{[1
]}

PARDI, A ^{[1
]}

机构：

[1] UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309

来源：

FEBS LETTERS | 1995年 / 362卷 / 02期

关键词：

HETERONUCLEAR NMRI RNA STRUCTURE; J COUPLING CONSTANT; BACKBONE TORSION ANGLE; RIBOZYME;

D O I：

10.1016/0014-5793(95)00232-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(3)J(CP) coupling constants have been measured in a 99% C-13,N-15 labeled lead-dependent ribozyme, known as the leadzyme, These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin-echo difference constant time HSQC experiment, This procedure permits improved measurement of the (3)J(C2'P) and (3)J(C4'P) coupling constants in isotopically labeled RNA and yielded valuable information on the beta and epsilon backbone torsion angles in the leadzyme.

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页码：156 / 160

页数：5

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