AMINO-ACIDS AND HUMAN AMNIOTIC-FLUID INCREASE DNA BIOSYNTHESIS IN PANCREATIC-ISLETS OF ADULT-MOUSE, BUT THIS EFFECT IS LOST FOLLOWING EXPOSURE TO STREPTOZOTOCIN

被引：13

作者：

DUNGER, A ^{[1
]}

SJOHOLM, A ^{[1
]}

EIZIRIK, DL ^{[1
]}

机构：

[1] CENT INST DIABET GERHARDT KATSCH,O-2201 KARLSBURG,GERMANY

来源：

PANCREAS | 1990年 / 5卷 / 06期

关键词：

Amino acids; Growth hormone; Human amniotic fluid; Insulin biosynthesis; Insulin release; Pancreatic islets; Polyamines; Streptozotocin;

D O I：

10.1097/00006676-199011000-00002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The present study describes the effects of growth hormone (GH), amino acids, and human amniotic fluid on the function and replication of normal and SZ-treated adult mouse pancreatic islets. Thus, mouse islets were exposed in vitro to SZ or vehicle only, and maintained in culture for 7 days in RPMI 1640 containing 11.1 mM glucose and the different supplements described above. Supplementation with amino acids increased the insulin accumulation in the medium, DNA biosynthesis, and polyamine contents in the control islets. In the SZ-treated islets, amino acids increased insulin accumulation in the medium and polyamine contents, but not DNA biosynthesis. Culture of control islets in the presence of 10% human amniotic fluid increased the insulin accumulation in the medium, islet insulin content, total protein and (pro)insulin biosynthesis, and DNA biosynthesis. However, in the SZ-treated islets, the effects of human amniotic fluid were limited to an increase in insulin content and insulin accumulation in the medium. GH significantly increased insulin accumulation in the medium in control, but not SZ-treated islets. In both groups of islets, GH failed to induce a significant increase in thymidine incorporation. It is concluded that amino acids and human amniotic fluid are potent stimulators of DNA biosynthesis in adult mouse pancreatic (3 cells, perhaps due to an increase in cellular polyamine contents. However, following exposure to streptozotocin, the islets are not anymore responsive to these stimulators of DNA biosynthesis. © 1990 Raven Press, New York.

引用

页码：639 / 646

页数：8

共 38 条

[1]

Eisenbarth G.S., Type I diabetes mellitus: A chronic autoim-mune disease, N Engl J Med, 314, pp. 1360-1368, (1986)

[2]

Gepts W., Pathologic anatomy of the pancreas in juvenile diabetes, Diabetes, 14, pp. 619-633, (1965)

[3]

Foulis A.K., The pathogenesis of beta cell destruction in type 1 (Insulin-dependent) diabetes mellitus, J Pathol, 152, pp. 141-148, (1987)

[4]

Hellerstrom C., Swenne I., Ersson A., Islet cell replication and diabetes, The Pathology of the Endocrine Pancreas in Diabetes, pp. 141-170, (1988)

[5]

Eizirik D.L., Sandler S., Function and metabolism of pancreatic β-cells maintained in culture following experimentally induced damage, Pharmacol Toxicol, 65, pp. 163-168, (1989)

[6]

Eizirik D.L., Sandler S., Welsh N., Hellerstrom C., Preferential reduction of insulin production in mouse pancreatic islets maintained in culture after streptozotocin exposure, Endo-Crinology, 122, pp. 1242-1249, (1988)

[7]

Strandell E., Eizirik D.L., Korsgren O., Sandler S., Functional characteristics of cultured mouse pancreatic islets following exposure to different streptozotocin concentrations, Mol Cell Endocrinol, 59, pp. 83-91, (1988)

[8]

Reiher H., Joschko H., Jentsch K., Et al., In vitro Untersuchugen von Fruchtwasser und intrauterine Retardierung, Zent Gynakol, 109, pp. 1191-1194, (1987)

[9]

Hehmke B., Kohnert K.D., Odeseilius R., The use of a new dextran gradient medium for rapid isolation of functionally intact neonatal rat pancreatic islets, Diabetes Res, 3, pp. 13-16, (1986)

[10]

Andersson A., Isolated mouse pancreatic islets in culture: Effects of serum and different culture medium on the insulin production of the islets, Diabetologia, 14, pp. 397-404, (1978)

← 1 2 3 4 →