RAPID SOLID-PHASE EXTRACTION OF DOPAMINE, SEROTONIN, AND THEIR ACIDIC METABOLITES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：1

作者：

CROWE, TD

JACOBSEN, DW

机构：

[1] The Cleveland Clinic Foundation, Department of Brain and Vascular Research, Cleveland

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1080/10826079208017166

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid solid-phase extraction procedure utilizing C18 reverse-phase mini-columns has been established for dopamine, 3,4-dihydroxyphenylacetic acid, serotonin, and 5-hydroxyindoleacetic acid in tissue perfusate samples. The method rapidly separates the analytes from protein in albumin-containing tissue perfusates. The analytes which are recovered in high yield are then determined by high-performance liquid chromatography with electrochemical detection. The mean interassay coefficient of variation for the recovery of dopamine, 3,4-dihydroxyphenylacetic acid, serotonin and 5-hydroxyindoleacetic acid was 12, 7, 13 and 8 percent, respectively.

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页码：221 / 230

页数：10

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