STUDY OF REVERSED-PHASE C-18-SILICA IN LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF FREE DISSOLVED AMINO-ACIDS AND COPPER(II) AMINO-ACID COMPLEXES AT THE PICOMOLE LEVEL IN MARINE MATRICES

被引：8

作者：

BAFFI, F ^{[1
]}

IANNI, MC ^{[1
]}

CARDINALE, AM ^{[1
]}

MAGI, E ^{[1
]}

FRACHE, R ^{[1
]}

RAVERA, M ^{[1
]}

机构：

[1] ITIS,I-16126 GENOA,ITALY

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 260卷 / 01期

关键词：

LIQUID CHROMATOGRAPHY; AMINO ACIDS; COPPER; SEA WATER; WATERS;

D O I：

10.1016/0003-2670(92)80132-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

With a view to future application to real sea-water samples, chromatographic conditions were investigated for studying the behavior of copper(II)-amino acid complexes on reversed-phase C18. Both direct injection and precolumn derivatization with omicron-phthalaldehyde of synthetic samples were considered. The final procedure achieved the complete separation of alpha-amino acids (free and metal-bound) after sample treatment and derivatization with omicron-phthaladehyde. The determination of copper(II)-amino acid complexes and 100% recovery of all copper species present in samples seem to be possible. The results provide information on the dissolved copper distribution in sea water.

引用

页码：99 / 106

页数：8

共 37 条

[1] HPLC DETERMINATION OF ALPHA-AMINO-ACIDS IN PHYTOPLANKTONIC MARINE CELLS [J].