MODEL INVARIANT METHOD FOR EXTRACTING SINGLE-CHANNEL MEAN OPEN AND CLOSED TIMES FROM HETEROGENEOUS MULTICHANNEL RECORDS

被引:21
作者
RAMANAN, SV
FAN, SF
BRINK, PR
机构
[1] Department of Physiology and Biophysics, State University of New York, Stony Brook
关键词
CHANNELS; OPEN TIME; CLOSED TIME; MEAN TIMES; INVARIANCE; HETEROGENEITY;
D O I
10.1016/0165-0270(92)90139-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We present a proof that the mean open (and closed) times of the individual channels in a multichannel record can be found in a model-independent fashion. As the results are model independent, they can be derived by assuming the simplest model for all the channels, namely that they all have the basic CLOSED half arrow right over half arrow left OPEN scheme. In particular, the method can be applied to patches where the channel population is heterogenous with respect to open probability. Multichannel simulations are performed to test the limits of applicability of this method to restricted amounts of data. One conclusion is that increasing the number of channels does not substantially reduce the errors in estimating the mean times, in spite of the 'increased information' present. We also prove the general applicability of the algorithm of Fenwick et al. (1982) in estimating the mean times without knowledge of the number of channels present, and discuss its limitations. An illustration using experimental data is also given.
引用
收藏
页码:91 / 103
页数:13
相关论文
共 19 条
[1]   CORRECTING SINGLE CHANNEL DATA FOR MISSED EVENTS [J].
BLATZ, AL ;
MAGLEBY, KL .
BIOPHYSICAL JOURNAL, 1986, 49 (05) :967-980
[2]   PATCH CLAMP RECORDINGS FROM MEMBRANES WHICH CONTAIN GAP JUNCTION CHANNELS [J].
BRINK, PR ;
FAN, SF .
BIOPHYSICAL JOURNAL, 1989, 56 (03) :579-593
[3]   RELATIONSHIP BETWEEN MEMBRANE EXCITABILITY AND SINGLE CHANNEL OPEN-CLOSE KINETICS [J].
CLAY, JR ;
DEFELICE, LJ .
BIOPHYSICAL JOURNAL, 1983, 42 (02) :151-157
[4]   ON THE STOCHASTIC PROPERTIES OF SINGLE ION CHANNELS [J].
COLQUHOUN, D ;
HAWKES, AG .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1981, 211 (1183) :205-235
[5]  
Colquhoun D., 1983, SINGLE CHANNEL RECOR, Vsecond, P191
[6]   YET ANOTHER APPROACH TO THE DWELL-TIME OMISSION PROBLEM OF SINGLE-CHANNEL ANALYSIS [J].
CROUZY, SC ;
SIGWORTH, FJ .
BIOPHYSICAL JOURNAL, 1990, 58 (03) :731-743
[7]  
DABROWSKI AR, 1990, ANN STAT, V18, P1111
[8]   SODIUM AND CALCIUM CHANNELS IN BOVINE CHROMAFFIN CELLS [J].
FENWICK, EM ;
MARTY, A ;
NEHER, E .
JOURNAL OF PHYSIOLOGY-LONDON, 1982, 331 (OCT) :599-635
[9]  
Fredkin D., 1985, P BERKELEY C HONOR J, V1, P269
[10]   ON AGGREGATED MARKOV-PROCESSES [J].
FREDKIN, DR ;
RICE, JA .
JOURNAL OF APPLIED PROBABILITY, 1986, 23 (01) :208-214