EFFECTS OF PROMOTERS ON THE ENHANCEMENT OF PECTIN METHYL ESTERASE EXPRESSION IN ASPERGILLUS-NIGER

被引：6

作者：

KHANH, NQ ^{[1
]}

LEIDINGER, K ^{[1
]}

ALBRECHT, H ^{[1
]}

RUTTKOWSKI, E ^{[1
]}

GOTTSCHALK, M ^{[1
]}

机构：

[1] ROHM GMBH,W-6100 DARMSTADT,GERMANY

来源：

BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 11期

关键词：

D O I：

10.1007/BF01021057

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pectin methylesterase-encoding gene (pmeA) has been cloned and transformed into A. niger wild-type NRRL3. Transformants produced 20-fold more PME than the host strain. For studying the effects of different promoters on the pmeA expression two novel plasmids were constructed, in which the pmeA promoter was replaced by efficient promoters such as the A. nidulans glyceraldehyde-3-phosphate dehydrogenase (pK45) or the A. oryzae alpha-amylase (pK61) promoter. The highest level of PME expression was achieved with the A. oryzae alpha-amylase promoter, reaching a 200-fold increase compared to the production by the host strain.

引用

页码：1047 / 1052

页数：6

共 9 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] ISOLATION OF GENOMIC CLONES CONTAINING THE AMDS GENE OF ASPERGILLUS-NIDULANS AND THEIR USE IN THE ANALYSIS OF STRUCTURAL AND REGULATORY MUTATIONS [J].