DENATURING AGENTS (UREA, ACRYLAMIDE) PROTECT ENZYMES AGAINST IRREVERSIBLE THERMO-INACTIVATION - STUDY WITH NATIVE AND IMMOBILIZED ALPHA-CHYMOTRYPSIN AND TRYPSIN

被引：32

作者：

MARTINEK, K ^{[1
]}

GOLDMACHER, VS ^{[1
]}

KLIBANOV, AM ^{[1
]}

BEREZIN, IV ^{[1
]}

机构：

[1] LOMONOSOV STATE UNIV,LAB BIOORG CHEM,A BLDG,MOSCOW 117234,USSR

来源：

FEBS LETTERS | 1975年 / 51卷 / 01期

关键词：

D O I：

10.1016/0014-5793(75)80874-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：152 / 155

页数：4

共 17 条

[1]

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CHASE, T ;

SHAW, E .

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[4] TRYPSINOGEN TRYPSIN TRYPSIN-SUBSTRATE AND TRYPSIN-INHIBITOR COMPLEXES IN UREA SOLUTIONS [J].

DELAAGE, M ;

LAZDUNSKI, M .

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[5]

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[6]

JOLY M, 1965, PHYSIOCHEMICAL APPRO

[7] SOME FACTORS IN THE INTERPRETATION OF PROTEIN DENATURATION [J].

KAUZMANN, W .

ADVANCES IN PROTEIN CHEMISTRY, 1959, 14 :1-63

[8] ETUDE STRUCTURALE DU TRYPSINOGENE ET DE LA TRYPSINE . LES DIAGRAMMES DETAT [J].

LAZDUNSKI, M ;

DELAAGE, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 140 (03) :417-+

[9] CONFORMATION CHANGES OF PROTEINS [J].

LUMRY, R ;

EYRING, H .

JOURNAL OF PHYSICAL CHEMISTRY, 1954, 58 (02) :110-120

[10]

Lumry R., 1969, STRUCTURE STABILITY, P65

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