DISTRIBUTION OF NITRATE REDUCTASE-ACTIVITY IN NODULATED LUCERNE PLANTS

被引：14

作者：

ARRESEIGOR, C ^{[1
]}

GARCIAPLAZAOLA, JI ^{[1
]}

DIAZ, A ^{[1
]}

APARICIOTEJO, PM ^{[1
]}

机构：

[1] UNIV BASQUE COUNTRY, DEPT BIOL VEGETAL & ECOL, E-48080 BILBAO, SPAIN

来源：

PLANT AND SOIL | 1991年 / 131卷 / 01期

关键词：

LUCERNE; NITRATE; NITRATE REDUCTASE; RHIZOBIUM-MELILOTI;

D O I：

10.1007/BF00010425

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nitrogen fixing plants of lucerne (Medicago sativa L. cv. Aragon) were grown in a glasshouse for three months in the absence of nitrate, and then supplied with 5 mM KNO3 for a week. In control (non-nitrate fed) plants, nitrate reductase activity (NRA EC 1.6.6.1) was detected only in nodules. After nitrate supply, root NRA showed a transient increase. Shoot NRA increased with time, paralleling changes in nitrate distribution; stem NRA represented nearly 50% of total NRA in plant tissues. Total nitrogen, expressed on a dry weight basis, tended to decrease in shoots upon nitrate supply. Bacteroid NRA (EC 1.7.99.4) showed a great variation depending on Rhizobium meliloti strains, ranging from 5 to 40% of total plant NRA. However, different Rhizobium strains did not give different results in terms of plant growth parameters, nitrate or organic nitrogen content.

引用

页码：107 / 113

页数：7

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