A TRANSITION-METAL COMPLEX (VENUS FLYTRAP CLUSTER) FOR RADIOIMMUNODETECTION AND RADIOIMMUNOTHERAPY

被引：54

作者：

PAXTON, RJ

BEATTY, BG

HAWTHORNE, MF

VARADARAJAN, A

WILLIAMS, LE

CURTIS, FL

KNOBLER, CB

BEATTY, JD

SHIVELY, JE

机构：

[1] CITY HOPE NATL MED CTR,DEPT GEN ONCOL SURG,DUARTE,CA 91010

[2] CITY HOPE NATL MED CTR,DEPT DIAGNOST RADIOL,DUARTE,CA 91010

[3] UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM,LOS ANGELES,CA 90024

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 08期

关键词：

CARBORANE; RADIONUCLIDES; COLON CANCER; CARCINOEMBRYONIC ANTIGEN; MONOCLONAL ANTIBODY;

D O I：

10.1073/pnas.88.8.3387

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel transition metal complex, Venus flytrap cluster (VFC), is described for the preparation of radiolabeled antibodies. VFC contained Co-57, which was held tightly between the faces of two covalently bridged carborane ligands by cluster bonding of the metal with appropriate ligand orbitals. Anti-carcinoembryonic antigen monoclonal antibody T84.66 was conjugated to Co-57-VFC with full retention of immunological activity. Biodistribution studies in nude mice bearing carcinoembryonic antigen-producing tumors showed excellent tumor localization of Co-57-VFC-T84.66. The accumulation of radionuclide in normal liver was low and independent of dose, which may reflect the stability of the radionuclide complex. These results presage the use of VFC systems for binding transition metals that are clinically useful for radioimmunodiagnosis and radioimmunotherapy.

引用

页码：3387 / 3391

页数：5

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