MAGNETIC-RESONANCE STUDIES OF MURINE MACROPHAGES - PROLIFERATION IS NOT A PREREQUISITE FOR ACQUISITION OF AN ACTIVATED HIGH-RESOLUTION SPECTRUM

Proton magnetic resonance spectroscopy (H-1-MRS) was used to investigate the membranes of macrophages activated by gamma-interferon in vitro and by Listeria monocytogenes in vivo. We report the appearance with activation, of a high resolution spectrum indistinguishable from that found in activated T and B cells and embryonic and malignant cell types previously studied. We furthermore show that proliferation is not a prerequisite for the appearance of this activated spectrum. This supports the idea that membrane 'activation' in all cells, irrespective of origin, may be accompanied by similar architectural changes, and suggests that a common pathway exists for the activation of cell membranes of the immune system, possibly important in the acquisition of increased motility. The use of H-1-MRS as a non-invasive tool for analysis of activation is discussed.