INVIVO ACTIVATION OF AN DELTA-6 OXYGENASE IN HUMAN SKIN

被引：29

作者：

BAER, AN ^{[1
]}

COSTELLO, PB ^{[1
]}

GREEN, FA ^{[1
]}

机构：

[1] VET AFFAIRS MED CTR,BUFFALO,NY 14215

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81260-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To test the hypothesis that an epidermal fatty acid oxygenase is activated in vivo under physiologic conditions, surface lipids from normal human skin were analyzed for oxygenase products. With high-performance liquid chromatography on reversed-phase and straight-phase chiral columns and gas-liquid chromatography/mass spectrometry, these lipids were found to contain free 13-hydroxyoctadeca-9Z,11E-dienoic acid and 9-hydroxyoctadeca-10E,12Z-dienoic acid. The 13-hydroxyoctadecadienoic acid was present as a stereoisomeric mixture, with an average S/R ratio of 2.2, and exceeded the concentration of 9-hydroxyoctadecadienoic acid by a factor of 2. These observations and others indicate that the 13-hydroxyoctadecadienoic acid was derived mostly from an ω-6 oxygenase (probably 15-lipoxygenase) which is activated in vivo in normal skin. © 1991 Academic Press, Inc.

引用

页码：98 / 104

页数：7

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