ORDERED ASSEMBLY OF A FUNCTIONAL PREINITIATION TRANSCRIPTION COMPLEX, CONTAINING VACCINIA VIRUS EARLY TRANSCRIPTION FACTOR AND RNA-POLYMERASE, ON AN IMMOBILIZED TEMPLATE

被引：19

作者：

BALDICK, CJ ^{[1
]}

CASSETTI, MC ^{[1
]}

HARRIS, N ^{[1
]}

MOSS, B ^{[1
]}

机构：

[1] NIAID,VIRAL DIS LAB,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 09期

关键词：

D O I：

10.1128/JVI.68.9.6052-6056.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A functional preinitiation transcription complex was formed by incubating vaccinia virus early transcription factor VETF and RAP94(+) RNA polymerase with an early promoter template immobilized on paramagnetic particles. A preferred order of assembly, VETF followed by RNA polymerase, was demonstrated by stepwise addition experiments. ATP was unnecessary for preinitiation transcription complex formation, but divalent cations were required specifically for the association of RNA polymerase.

引用

页码：6052 / 6056

页数：5

共 45 条

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