THE MOLECULAR DIVERSITY OF THE 5S RIBOSOMAL-RNA GENE IN BARLEY (HORDEUM-VULGARE)

被引:46
作者
BAUM, BR [1 ]
JOHNSON, DA [1 ]
机构
[1] UNIV OTTAWA,OTTAWA CARLETON INST BIOL,OTTAWA,ON K1N 6N5,CANADA
关键词
5S RDNA; SEQUENCE DIVERSITY; BARLEY; PHYLOGENETIC ANALYSIS;
D O I
10.1139/g94-140
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 5S rDNA genes from several accessions of cultivated barley, Hordeum vulgare L., were amplified by the polymerase chain reaction, cloned, and sequenced. Analysis of the aligned sequences, followed by principal coordinate analysis, support the recognition of at least two distinct classes of 5S rDNA genes. The short repeat class corresponds to the 300-bp tandem repeat defined by E.V. Ananiev as containing several TAG repeating units. The long repeat class contains long tandem repeats and lacks the TAG repeating unit. Sequences in each class can be further subdivided, with the long repeat class containing two groups and the short repeat class containing two and possibly three groups. These results suggest that in cultivated barley the sequence diversity found within the 5S rDNA nontranscribed spacer region may be encoded by three or more loci and may be useful for phylogenetic analyses provided that orthology can be established.
引用
收藏
页码:992 / 998
页数:7
相关论文
共 31 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] ANANIEV EV, 1990, BIOTECHNOLOGY AGR, V5, P133
  • [3] THE 5S-DNA UNITS OF BREAD WHEAT (TRITICUM-AESTIVUM)
    APPELS, R
    BAUM, BR
    CLARKE, BC
    [J]. PLANT SYSTEMATICS AND EVOLUTION, 1992, 183 (3-4) : 183 - 194
  • [4] MOLECULAR AND CHROMOSOMAL ORGANIZATION OF DNA-SEQUENCES CODING FOR THE RIBOSOMAL-RNAS IN CEREALS
    APPELS, R
    GERLACH, WL
    DENNIS, ES
    SWIFT, H
    PEACOCK, WJ
    [J]. CHROMOSOMA, 1980, 78 (03) : 293 - 311
  • [5] APPELS R, 1991, MOL PLANT SYSTEMATIC, P92
  • [6] EVOLUTIONARY CHANGE AT THE 5S-DNA LOCI OF SPECIES IN THE TRITICEAE
    BAUM, BR
    APPELS, R
    [J]. PLANT SYSTEMATICS AND EVOLUTION, 1992, 183 (3-4) : 195 - 208
  • [7] BAUM BR, 1994, IN PRESS PLANT MOL B
  • [8] CLARK SP, 1992, COMPUT APPL BIOSCI, V8, P535
  • [9] DERIJK P, 1994, IN PRESS COMPUT APPL
  • [10] PHYSICAL MAPPING OF 5S RDNA LOCI BY DIRECT-CLONED BIOTINYLATED PROBES IN BARLEY CHROMOSOMES
    FUKUI, K
    KAMISUGI, Y
    SAKAI, F
    [J]. GENOME, 1994, 37 (01) : 105 - 111