FINDING PROSPECTIVE PARTNERS IN THE LIBRARY - THE 2-HYBRID SYSTEM AND PHAGE DISPLAY FIND A MATCH

被引：83

作者：

ALLEN, JB

WALBERG, MW

EDWARDS, MC

ELLEDGE, SJ

机构：

[1] BAYLOR COLL MED,VERNA & MARRS MCLEAN DEPT BIOCHEM,HOUSTON,TX 77030

[2] BAYLOR COLL MED,DEPT HUMAN & MOLEC GENET,HOUSTON,TX 77030

[3] UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED,DALLAS,TX 75235

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1995年 / 20卷 / 12期

关键词：

D O I：

10.1016/S0968-0004(00)89119-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The two-hybrid system uses the efficacy of yeast genetic assays to identify protein-protein interactions. It permits the rapid cloning of genes encoding products that interact with a given protein of interest. Also being developed are phage display methods that allow direct physical selection of binding proteins. These methods have significantly altered strategies for analysing signaling and regulatory pathways.

引用

页码：511 / 516

页数：6

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