DYNAMICS OF PORPHYRIN MOLECULES IN MICELLES - PICOSECOND TIME-RESOLVED FLUORESCENCE ANISOTROPY STUDIES

被引:88
作者
MAITI, NC [1 ]
MAZUMDAR, S [1 ]
PERIASAMY, N [1 ]
机构
[1] TATA INST FUNDAMENTAL RES,CHEM PHYS GRP,BOMBAY 400005,MAHARASHTRA,INDIA
关键词
D O I
10.1021/j100027a006
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The fluorescence dynamics of protoporphyrin IX (PPIX) and protoporphyrin IX dimethyl ester (PPDME) and their zinc complexes in anionic (SDS), cationic (CTAB), and neutral (Triton X-100) micelles have been studied by picosecond time-resolved fluorescence and anisotropy decay measurements using a time-correlated single photon counting technique. The absorption and fluorescence emission spectra of the porphyrins (0.5-1 mu M) in the micelles (0.5-1 mM) are typical of the monomeric porphyrin. The absence of aggregation and homogeneity of the solubilization sites are confirmed by the fluorescence decay which is a single exponential for all the four porphyrins in the neutral and cationic micelles, Triton X-100 and CTAB. However, the presence of small. concentrations of aggregates resulted in biexponential fluorescence decay in the anionic SDS micelles. The fluorescence anisotropy decay of the monomer-micelle systems is best fitted to a two-exponential function. The values of the rotational decay parameters are consistent with the model that the fluorescence depolarization occurs by both rotational and translational diffusion of the porphyrin inside the micelle and the tumbling of the micelle. The order parameter for the porphyrin intercalation and rotational and translational diffusion coefficients have been calculated. The model is consistent with the NMR results which show that the equilibrium distribution of porphyrin is confined to a region near the surface.
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页码:10708 / 10715
页数:8
相关论文
共 47 条
[1]  
[Anonymous], 1969, DATA REDUCTION ERROR
[2]   DYNAMICS OF FLUORESCENCE POLARIZATION IN MACROMOLECULES [J].
BELFORD, GG ;
BELFORD, RL ;
WEBER, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1392-&
[3]   LIGHT-SCATTERING FROM POLYDISPERSE SDS MICELLAR SOLUTIONS [J].
BRIGGS, J ;
NICOLI, DF ;
CICCOLELLO, R .
CHEMICAL PHYSICS LETTERS, 1980, 73 (01) :149-152
[5]   ROTATIONAL DIFFUSION OF ACRIDINE-ORANGE ATTACHED TO SDS MICELLES [J].
CHOU, SH ;
WIRTH, MJ .
JOURNAL OF PHYSICAL CHEMISTRY, 1989, 93 (22) :7694-7698
[6]   THEORY OF FLUORESCENCE DEPOLARIZATION BY ANISOTROPIC ROTATIONAL DIFFUSION [J].
CHUANG, TJ ;
EISENTHAL, KB .
JOURNAL OF CHEMICAL PHYSICS, 1972, 57 (12) :5094-+
[7]   PHOTOCHEMICAL REACTIVITY IN ORGANIZED ASSEMBLIES .30. SELF-SENSITIZED PHOTO-OXIDATION OF PROTOPORPHYRIN-IX DERIVATIVES IN AQUEOUS SURFACTANT SOLUTIONS - PRODUCT AND MECHANISTIC STUDIES [J].
COX, GS ;
KRIEG, M ;
WHITTEN, DG .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (25) :6930-6937
[8]   ROTATIONAL REORIENTATION DYNAMICS OF POLAR DYE MOLECULAR PROBES BY PICOSECOND LASER SPECTROSCOPIC TECHNIQUE [J].
DUTT, GB ;
DORAISWAMY, S ;
PERIASAMY, N ;
VENKATARAMAN, B .
JOURNAL OF CHEMICAL PHYSICS, 1990, 93 (12) :8498-8513
[9]   MOLECULAR-REORIENTATION DYNAMICS OF POLAR DYE PROBES IN TERTIARY-BUTYL ALCOHOL WATER MIXTURES [J].
DUTT, GB ;
DORAISWAMY, S ;
PERIASAMY, N .
JOURNAL OF CHEMICAL PHYSICS, 1991, 94 (08) :5360-5368
[10]   MOLECULAR VOLUMES AND STOKES-EINSTEIN EQUATION [J].
EDWARD, JT .
JOURNAL OF CHEMICAL EDUCATION, 1970, 47 (04) :261-&