O-GLYCOSIDICALLY LINKED N-ACETYLGLUCOSAMINE-BOUND OLIGOSACCHARIDES FROM GLYCOPROTEINS OF TRYPANOSOMA-CRUZI

被引：100

作者：

PREVIATO, JO

JONES, C

GONCALVES, LPB

WAIT, R

TRAVASSOS, LR

MENDONCAPREVIATO, L

机构：

[1] NATL INST BIOL STAND & CONTROLS,MOLEC STRUCT LAB,POTTERS BAR EN6 3QG,HERTS,ENGLAND

[2] FED UNIV RIO DE JANEIRO,INST MICROBIOL CCS BLOCO 1,BR-21944970 RIO JANEIRO,RJ,BRAZIL

[3] EPM,DISCIPLINA BIOL CELULAR,BR-04023062 SAO PAULO,BRAZIL

[4] PUBL HLTH LAB SERV,DIV PATHOL,SALISBURY SP4 0JG,WILTS,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1994年 / 301卷

关键词：

D O I：

10.1042/bj3010151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this report we describe studies on the structures of the O-linked carbohydrate units in cell-surface glycoproteins of epimastigote forms of the G-strain of Trypanosoma cruzi. Mild alkaline reductive degradation of the 38/43 kDa glycoproteins resulted in beta-elimination of glycosylated threonine and/or serine residues, and the liberation of N-acetylglucosaminitol, galactobiosyl-, galactotriosyl-, galactotetraosyl and galacto-pentaosyl-N-acetylglucosaminitol. The structures of these oligosaccharide alditols were established by n.m.r. spectroscopy and methylation analysis as: Galf beta 1-4(Galp beta 1-6)GlcNAc-ol Galp beta 1-3Galp beta 1-6(Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galp beta 1 -2Galf beta 1-4)GlcNAc-ol.

引用

页码：151 / 159

页数：9

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